Determination of selenium after derivatization with 2,3-diaminonaphtalene in water and serum

By wet treatment with chemicals and reduction, selenium is conversed to selenite and derivatized to 2,1,3-naphtoselenodiazol. It is then extracted by Extrelut-columns. The eluate is chromatographed on silica with chloroform. To increase fluorescence intensity, the plate is dipped in a solution of Triton X-100 and then evaluated quantitatively by fluorescence measurement.

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