Determination of vitamin B1 in pharmaceutical products

The method is suitable for vitamin B1 (thiamine hydrochloride) determination in quality assurance, stability tests, etc. The methanolic solution of the drug is chromatographed on silica with methanol – NH3 -acetic acid – chloroform 18:2:1:1, then derivatized with potassium hexacyanoferrate. After stabilising the fluorescence intensity with paraffin oil the plate is evaluated by densitometry. The determination limit is 500 pg/spot.

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