Chiral separation of transstilbene oxide using SFC

Chiral SFC mostly uses the same columns as chiral High-Performance Liquid Chromatography (HPLC), with the most popular being the polysaccharide chiral stationary phases (CSPs), which are highly versatile due to the selection of differently modified polysaccharides [3]. Polysaccharide CSPs have a high loading capacity, which makes them useful in preparative-scale applications. Numerous commercial polysaccharide chiral stationary phases are available, primarily based on amylose or cellulose and modified with halogenated or non-halogenated aromatic groups. The modified polysaccharide can be either coated or immobilized onto the silica support to enhance its resistance to strong solvents [3]. There are other CSPs that are often used for chiral SFC applications, e.g., the Pirkle phases [3].

This application note describes the chiral column screening with trans-stilbene oxide as a chiral molecule and the consecutive implementation of a preparative method. This was followed by the creation of a stacked injection method and a preparative separation.